Description
The multispecies 3-Nitrotyrosine (3-NT) ELISA quantitates 3-NT in serum, plasma, and other biological fluids
Principle of the method:
The Competitive multispecies ELISA research-use-only kit is designed to quantitatively measure 3-Nitrotyrosine (3-NT) independent of species. A 3-NT standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to 3-NT is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of 3-NT in the samples is then determined by comparing the OD of the samples to the standard curve.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation